W. J. Nicolas

MicroED structure of the human vasopressin 1B receptor

This paper determines the structure of the human vasopressin 1B receptor using MicroED, overcoming challenges in GPCR structural determination.

Design and implementation of suspended drop crystallization

This paper introduces a new crystal growth method called suspended drop crystallization, which allows for direct crystal growth on an EM grid and enables structure determination by MicroED.

A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals

This paper introduces a method to prepare lipidic cubic phase (LCP) membrane protein crystals for MicroED by using fluorescence microscopy to identify and mill crystals without converting LCP to sponge phase.

Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus

This study uses cryo-electron tomography to image the predation cycle of Bdellovibrio bacteriovorus, revealing structural adaptations during prey invasion and replication.

Bimodally oriented cellulose fibers and reticulated homogalacturonan networks - A direct visualization of Allium cepa primary cell walls

This study visualizes the ultrastructure of onion cell walls, revealing bimodal cellulose fiber orientation and HG-rich meshing networks.

Structure of the bacterial cellulose ribbon and its assembly-guiding cytoskeleton by electron cryotomography

This study uses cryo-electron tomography to reveal the structure of bacterial cellulose ribbons and a novel cytoskeletal structure called the cortical belt in Gluconacetobacter species.

Multiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata

This study identifies MCTPs as ER-PM tethers at plasmodesmata, linking membrane tethering to cell-to-cell signaling in plants.