Maike Thamsen Dunyak

This paper introduces L-PGI, a ligase-mediated gene editing system that enables efficient and precise genomic integration in both dividing and nondividing cells, overcoming limitations of reverse transcriptase-based methods.

The paper identifies low fidelity in RT-based genome editing in non-dividing mouse hepatocytes and proposes solutions using neonatal mice and transgenic models.

The paper develops Bxb1 variants with improved integrase activity and fidelity, enhancing in vivo efficiency and reducing off-target effects.

This paper demonstrates programmable genomic integration in non-human primate liver, achieving curative levels of gene expression in over 50% of liver cells.