Jesse C. Cochrane

Ligase-mediated programmable genomic integration (L-PGI): an efficient site-specific gene editing system that overcomes the limitations of reverse transcriptase-based editing systems

This paper introduces L-PGI, a ligase-mediated gene editing system that enables efficient and precise genomic integration in both dividing and nondividing cells, overcoming limitations of reverse transcriptase-based methods.

Breaking Free: Development of Circular AAV Cargos for Targeted Seamless Integration in the Liver

This paper develops circular AAV vectors for DSB-free genomic integration in the liver, demonstrating seamless gene insertion in human hepatocytes and in vivo.

Engineered Bxb1 variants improve integrase activity and fidelity

The paper develops Bxb1 variants with improved integrase activity and fidelity, enhancing in vivo efficiency and reducing off-target effects.

Curative levels of endogenous gene replacement achieved in non-human primate liver using programmable genomic integration

This paper demonstrates programmable genomic integration in non-human primate liver, achieving curative levels of gene expression in over 50% of liver cells.

Large Serine Integrase Off-target Discovery and Validation for Therapeutic Genome Editing

The paper develops and validates methods to detect off-target recombination by Large Serine Integrases, finding higher sensitivity than whole genome sequencing.