Jenny Xie

Ligase-mediated programmable genomic integration (L-PGI): an efficient site-specific gene editing system that overcomes the limitations of reverse transcriptase-based editing systems

This paper introduces L-PGI, a ligase-mediated gene editing system that enables efficient and precise genomic integration in both dividing and nondividing cells, overcoming limitations of reverse transcriptase-based methods.

Breaking Free: Development of Circular AAV Cargos for Targeted Seamless Integration in the Liver

This paper develops circular AAV vectors for DSB-free genomic integration in the liver, demonstrating seamless gene insertion in human hepatocytes and in vivo.

Low RT-based Genome Editing Fidelity in Mouse Hepatocytes: Challenges and Solutions

The paper identifies low fidelity in RT-based genome editing in non-dividing mouse hepatocytes and proposes solutions using neonatal mice and transgenic models.

Curative levels of endogenous gene replacement achieved in non-human primate liver using programmable genomic integration

This paper demonstrates programmable genomic integration in non-human primate liver, achieving curative levels of gene expression in over 50% of liver cells.

Large Serine Integrase Off-target Discovery and Validation for Therapeutic Genome Editing

The paper develops and validates methods to detect off-target recombination by Large Serine Integrases, finding higher sensitivity than whole genome sequencing.