Jessica R. Von Stetina

This paper introduces L-PGI, a ligase-mediated gene editing system that enables efficient and precise genomic integration in both dividing and nondividing cells, overcoming limitations of reverse transcriptase-based methods.

This paper develops circular AAV vectors for DSB-free genomic integration in the liver, demonstrating seamless gene insertion in human hepatocytes and in vivo.

The paper identifies low fidelity in RT-based genome editing in non-dividing mouse hepatocytes and proposes solutions using neonatal mice and transgenic models.

The paper develops Bxb1 variants with improved integrase activity and fidelity, enhancing in vivo efficiency and reducing off-target effects.

The paper develops long-read sequencing assays to detect on- and off-target integration of rAAV vectors in human genomic DNA, finding no evidence of off-target integration.