Brett Estes

This paper introduces L-PGI, a ligase-mediated gene editing system that enables efficient and precise genomic integration in both dividing and nondividing cells, overcoming limitations of reverse transcriptase-based methods.

This paper develops circular AAV vectors for DSB-free genomic integration in the liver, demonstrating seamless gene insertion in human hepatocytes and in vivo.

The paper identifies low fidelity in RT-based genome editing in non-dividing mouse hepatocytes and proposes solutions using neonatal mice and transgenic models.

This paper demonstrates programmable genomic integration in non-human primate liver, achieving curative levels of gene expression in over 50% of liver cells.

The paper develops and validates methods to detect off-target recombination by Large Serine Integrases, finding higher sensitivity than whole genome sequencing.