O. Gandrillon

In silico single-cell metabolism analysis unravels a new transition stage of CD8 T cells 4 days post-infection

The paper uses in silico analysis of single-cell RNA-seq data to identify a new metabolic transition stage in CD8 T cells 4 days post-infection.

An Image-Guided Microfluidic System for Single-Cell Lineage Tracking

This paper introduces a microfluidic system for single-cell lineage tracking, demonstrating its ability to isolate and analyze sister cells with high viability for downstream RNA sequencing.

Differentiation is accompanied by a progressive loss in transcriptional memory

The paper quantifies how transcriptional memory decreases during differentiation by comparing related and unrelated cells across generations.

CD8 memory precursor cells generation is a continuous process

The paper identifies that memory precursor cells are continuously generated over time following acute infection, with increased numbers and effector gene expression observed later.

Evidence for close molecular proximity between reverting and undifferentiated cells

The paper characterizes reverting cells by comparing their molecular state to undifferentiated cells using single-cell transcriptomic data and finds they return to an earlier molecular state with minimal differences.

Modeling and Characterization of Inter-Individual Variability in CD8 T Cell Responses in Mice

The paper develops a dynamical model to analyze inter-individual variability in CD8 T cell responses in mice across different immunization contexts.

Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

This study shows that erythroid differentiation involves a metabolic switch from glycolysis to oxidative phosphorylation, with a peak of energy consumption at 12-24h.

Model-based assessment of the Role of Uneven Partitioning of Molecular Content on Heterogeneity and Regulation of Differentiation in CD8 T-cell Immune Responses

The paper uses a multiscale model to show that uneven molecular partitioning during CD8 T-cell division regulates differentiation and promotes memory cell generation.

Automated cell cycle and cell size measurements for single-cell gene expression studies

This paper introduces an automatic, toxic-free method to measure cell cycle and size in single-cell studies, showing minimal influence of these factors on gene expression variability in chicken erythroid progenitors.

Integrated time-lapse and single-cell transcription studies highlight the variable and dynamic nature of human hematopoietic cell fate commitment.

The paper integrates single-cell transcriptional data with time-lapse microscopy to show dynamic and variable hematopoietic cell fate commitment, revealing non-binary lineage decisions.