Engineered translation release factor 1 suppresses disease-causing nonsense mutations and enables multi-site nonstandard amino acids incorporation
Yuting Chen, Meilin Tian, Zhu Zhu, Qian Ya, Yichong Xu, Peng Gong, George McDonald Church, Chenli Liu
This paper introduces OPENER, an engineered eRF1 variant that suppresses nonsense mutations and enables nonstandard amino acid incorporation, showing therapeutic benefits in a Duchenne muscular dystrophy model.
bioRxiv · Dec 25, 2025 · PreprintSynthetic Biology