R. Mahadevan

From mec cassette to rdhA: a key Dehalobacter genomic neighborhood in a chloroform and dichloromethane-transforming microbial consortium

The paper identifies functional genes in a microbial consortium that dechlorinates chloroform to dichloromethane and mineralizes dichloromethane, using metagenomic and metaproteomic analysis.

Evaluating the feasibility of medium chain oleochemical synthesis using microbial chain elongation

This study evaluates the feasibility of expanding the product range of chain elongating bacteria to produce C9-C12 fatty acids and other derivatives using metabolic engineering and constraint-based modelling.

Understanding systems level metabolic adaptation resulting from osmotic stress

This study develops a novel modeling approach to analyze metabolic adaptation in E. coli under osmotic stress, revealing prioritization of thermodynamically favorable pathways.

A multifunctional Dehalobacter? Tandem chloroform and dichloromethane degradation in a mixed microbial culture

This paper demonstrates a mixed microbial culture that can simultaneously degrade chloroform and dichloromethane to carbon dioxide without exogenous electron donor, suggesting intraspecies electron transfer for CF respiration.

Engineering Escherichia coli to produce aromatic chemicals from ethylene glycol

This study engineers Escherichia coli to produce aromatic chemicals from ethylene glycol, achieving higher yields than glucose and demonstrating feasibility with waste PET-derived EG.

Quantifying metal ion specificity of the nickel-binding protein CcNikZ-II from Clostridium carboxidivorans in the presence of competing metal ions

The paper quantifies how the nickel-binding protein CcNikZ-II from Clostridium carboxidivorans binds different metal ions in the presence of competitors using a fluorescence quenching method and kinetic models.

Ni(II) binding affinity and specificity of solute binding proteins: the importance of the double His motif and variable loop revealed by structural and mutational studies

This paper investigates Ni(II) binding in solute binding proteins, showing the double His motif and variable loop are critical for affinity and specificity, with implications for biocatalytic applications.

Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

This study engineered Escherichia coli to enhance nickel bioaccumulation using overexpressed NikABCDE transporter and metallothionein, achieving 7-fold improvement but with reduced cell viability.

Genetic engineering of Acidithiobacillus ferridurans with CRISPR-Cas9/dCas9 systems

This paper develops two CRISPR-based systems for genetic manipulation in Acidithiobacillus ferridurans, achieving efficient gene knockdown and genome editing with significant effects on sulfur and iron metabolism.

REVOLVER: a low-cost automated protein purifier based on parallel preparative gravity column workflows

REVOLVER is a low-cost automated protein purification system that enables parallel processing with minimal human intervention, achieving results comparable to manual methods.

Context-Dependent Flux Coupling via Conserved Small-Molecule Regulatory Structures

The paper analyzes the Escherichia coli small-molecule regulatory network to show how conserved feedforward loops optimize metabolic controllability through flux coupling.

Automation assisted anaerobic phenotyping for metabolic engineering

This paper develops an eco-friendly automation workflow for high-throughput anaerobic phenotyping to support metabolic engineering.

Challenges in Undergraduate Synthetic Biology Training: Insights from a Canadian iGEM Student Perspective

This study surveys Canadian undergraduate synthetic biology education through iGEM team experiences to provide insights and recommendations for teaching practices.

A microplate screen to estimate metal-binding affinities of metalloproteins

The paper introduces a high-throughput microITFQ-LTA method to screen metal-binding affinities of metalloproteins and validates it using two nickel-specific SBPs.

Interspecies malate-pyruvate shuttle drives amino acid exchange in organohalide-respiring microbial communities

This study identifies an interspecies malate-pyruvate shuttle in microbial communities that enables amino acid exchange under redox constraints.