R. W. Cheloha

Rapid covalent labeling of a GPCR on living cells using a nanobody-epitope tag pair to interrogate receptor pharmacology

This paper introduces a method using a nanobody-epitope tag pair to rapidly covalently label GPCRs on live cells, enabling specific pharmacological studies.

Characterization of a nanobody-epitope tag interaction and its application for receptor engineering

The paper characterizes a nanobody-epitope interaction and uses it to enhance ligand potency at an engineered adenosine A2A receptor.

Engineering protease-resistant peptides to inhibit human parainfluenza viral respiratory infection

This paper develops protease-resistant lipopeptides to inhibit HPIV3 infection by modifying the HRC domain, showing improved in vivo stability and antiviral activity in animals.

Antigen-specific induction of CD4+CD8αα+ intraepithelial T lymphocytes byBacteroidetes species

This study identifies Bacteroidetes species that induce CD4+CD8αα+ intraepithelial T lymphocytes, which provide protective immunity against colitis.

A nanobody that recognizes a 14-residue peptide epitope in the E2 ubiquitin-conjugating enzyme UBC6e modulates its activity

The paper identifies a nanobody that binds to a 14-residue epitope on UBC6e, enhancing its enzymatic activity and inhibiting phosphorylation in response to cell stress.