Author

Ralf Kuehn

Max Delbrück Center

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 1 Genomics· 1

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Approach:experimental assay· 2 Research Focus:gene regulatory network inference· 2 Organism:human· 2 Research Focus:drug response / drug discovery· 1 Organism:mouse· 1 Research Focus:protein structure prediction· 1 Assay:proteomics· 1

Frequent Co-Authors

Alexandra Roman· 1 shared Ana-Maria NitulescuiD· 1 shared Anja Zimmer· 1 shared Annette Kuenkele· 1 shared Casper F. T. van der VeniD· 1 shared Chahrazad Lachiheb· 1 shared Claudia Marti Grifol· 1 shared Dimitrios L. WagneriD· 1 shared

Activity by Year

2026-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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1 shared paperGenomics

Alexandra Roman

Mutual co-authors in cluster

Author profile →

2 papers

Recent·Most cited

Efficient CRISPR/Cas9-mediated homology independent sequence replacement in vivo and non-dividing primary cells

Tu Dang, Alexandra Roman, Anja Zimmer, Mikhail Y. Lebedin, Ella Bahry, Claudia Marti Grifol, Markus Esser, Seren Sevim-Wunderlich, Duncan C. Miller, Sebastian Diecke, Ralf Kuehn

The paper introduces REPLACE, a CRISPR/Cas9-based method for homology-independent sequence replacement in non-dividing cells, achieving efficient gene and exon editing in primary cells and post-mitotic cardiomyocytes.

bioRxiv · Jul 24, 2026 · PreprintGenomics

A Non-Viral CRISPR/Cas9 HDR Platform for Stable Engineering of Solid Tumor Models.

Shoaib Afzal, Maximilian Pilgram, Julia Macos, Emily Ohlendorf, Lena Olivia Raab, Jonas Kath, Viktor Glaser, Ana-Maria Nitulescu, Casper F. T. van der Ven, Chahrazad Lachiheb, Maria Stecklum, Norman Michael Drzeniek, Kathleen Anders, Dimitrios L. Wagner, Ralf Kuehn, , Michael Launspach

This paper develops a non-viral CRISPR/Cas9 HDR platform for stable transgene knock-in in solid tumor models, achieving up to 60% efficiency in neuroblastoma cells.

bioRxiv · Jun 4, 2026 · PreprintBioengineering