Highly effective gene inactivation in tetraploid Xenopus laevis with low-temperature-active engineered Cas12a
S. Yun, S. Kim, J. Hong, S. Shin, J. Choi, J.-S. Shin, S. Choi, H.-S. Lee, S. W. Cho, T. J. Park, T. Kwon
This study evaluates engineered Cas12a variants for gene inactivation in Xenopus laevis, finding that Cas12a-Ultra achieves high efficiency at low temperatures.
bioRxiv · Jul 30, 2023 · PreprintBioengineering