Nanobody-mediated modulation of long RSH enzymes Rel and RelA catalysis by restriction of their conformational landscape
Katleen Van Nerom, Andres Ainelo, Kyo Coppieters ’t Wallant, Ariel Talavera, Dannele Echemendia-Blanco, Sarah Peeters, Brahim El Khalfaoui Oulali, Hedvig Tamman, Tatsuaki Kurata, Mohammad Roghanian, Chloé Martens, Els Pardon, Jan Steyaert, Vasili Hauryliuk, Abel García-Pino
This study identifies nanobodies that modulate Rel and RelA enzyme activity by trapping them in specific conformational states, inhibiting (p)ppGpp synthesis and affecting bacterial virulence.
bioRxiv · Aug 7, 2025 · PreprintMicrobiology