One-step generation of TCR knock-in mice targeted to the TCRβ locus results in functional mature T lymphocytes
Jana Bilanovic, Juliana Bortolatto, Susu Duan, Valgerdur Bjornsdottir, A. Katharina Teetz, Alexandra Thoms, Julian Fischbach, Fabian Schmidt, William A. Nyberg, Harald Hartweger, Amelia Escolano, Paul G. Thomas, Gabriel D. Victora, Angelina Morand Bianchi Bilate, Johanne Tracey Jacobsen
This paper develops a method to generate TCR knock-in mice with functional T cells by integrating TCRβ sequences into the Trb locus using AAV and CRISPR/Cas9, overcoming limitations of traditional transgenic models.
bioRxiv · Jan 14, 2026 · PreprintImmunology