William A. Nyberg

Development of a Fully Non-Viral 1XX-enhanced BCMA CAR-T Cell Therapy for Multiple Myeloma

This paper develops a non-viral BCMA CAR-T therapy using CRISPR and 1XX signaling, showing improved safety, potency, and persistence in multiple myeloma.

Discovery of a Linked Constellation of Gene Expression Revealed by Local Editing of Fibroblasts in Tumors

The paper uses local gene editing in mouse tumor models to discover a novel fibroblast state and its impact on the tumor microenvironment.

One-step generation of TCR knock-in mice targeted to the TCRβ locus results in functional mature T lymphocytes

This paper develops a method to generate TCR knock-in mice with functional T cells by integrating TCRβ sequences into the Trb locus using AAV and CRISPR/Cas9, overcoming limitations of traditional transgenic models.

Decoding Peripheral Tolerance: TCR Rules for pTreg differentiation in the Gut

The paper shows that TCRs from Tregs are more effective than Tconv TCRs in driving pTreg differentiation, with distinct phenotypes based on antigen type.

Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function

This paper develops a genome engineering workflow for primary human NK cells using CRISPR/Cas9 and AAV6 to integrate and disrupt genes, enhancing anti-tumor function through targeted CAR expression.

Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells

This study introduces hydroporation as a scalable and efficient method for delivering CAR-T cells, achieving higher yields and viability compared to traditional methods.

Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection

This paper develops a method to efficiently reprogram T cells at multiple loci using SEED-Selection, achieving high purity for single and combined gene edits.