MicrobiologybioRxiv
Pooled CRISPR interference screens enable high-throughput functional genomics study and elucidate new rules for guide RNA library design in Escherichia coli
T. Wang, J. Guo, C. Guan…·X.-H. Xing
This paper develops a CRISPRi library for high-throughput functional genomics in E. coli, identifies sgRNA design rules, and validates gene-phenotype associations with high specificity.
DOI 10.1038/s41467-018-04899-x