Giulia Franciosa

This study profiles the proteomics and phosphoproteomics of melanoma cells under T cell attack to identify immune checkpoint targets and therapeutic vulnerabilities.

The paper shows that a short IFN-γ pulse induces a long-term proteomic signature in melanoma cells, challenging assumptions about cytokine signaling duration.

The paper introduces hybrid-DIA, a method combining targeted and discovery phosphoproteomics for single spheroids, showing comparable accuracy to existing methods while profiling the global phosphoproteome.