Joshua C. Cofsky

The paper reports cryo-EM structures of GerA, revealing how nutrient binding triggers channel opening through allosteric switches and subunit interactions.

This paper shows YpeB inhibits SleB in dormant Bacillus subtilis spores through direct interaction, preventing premature cortex degradation.

This study identifies a trade-off between PAM recognition breadth and CRISPR-Cas9 editing efficiency, proposing a two-step target capture mechanism to improve genome editing.

The paper shows CRISPR-Cas12a bends DNA to destabilize base pairs during target recognition, similar to CRISPR-Cas9 but with different hybridization directionality.

The paper introduces multiAsCas12a, an engineered CRISPR-Cas12a variant enabling higher-order combinatorial chromatin perturbations, and demonstrates its use in discovering new enhancer elements and dissecting cis-regulatory element functions.