Fabian Lim

The paper benchmarks a high-efficiency prime editing platform for multiplexed dropout screening of genetic variants, identifying negative growth phenotypes for nonsense mutations and splice-disrupting synonymous mutations.

The paper identifies conserved notochord-specific enhancers of Brachyury/T/TBXT in vertebrates and shows their role in notochord expression and development.

The paper shows that optimizing low-affinity ETS binding sites in the ZRS enhancer causes limb development defects, with severity increasing with higher affinity gains.

The paper identifies ETS affinity-optimizing SNVs in heart enhancers that disrupt development and contribute to cardiac traits in Ciona and human iPSC-CMs.