Britt Adamson

Prime editing enables high-throughput identification and characterization of selective MutSβ inhibitors

This study uses prime editing to identify and characterize MutSβ inhibitors that reduce (CAG)n repeat expansion.

Efficient Genome Editing with Chimeric Oligonucleotide-Directed Editing

This paper introduces CODEMax and CODEMax(exo+) genome editing systems that enable efficient and precise search-and-replace editing in human cells with reduced off-target effects.

Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes

The paper investigates DNA repair in postmitotic neurons and shows that manipulating repair pathways can influence CRISPR editing outcomes.

A benchmarked, high-efficiency prime editing platform for multiplexed dropout screening

The paper benchmarks a high-efficiency prime editing platform for multiplexed dropout screening of genetic variants, identifying negative growth phenotypes for nonsense mutations and splice-disrupting synonymous mutations.

Mapping the Genetic Interaction Network of PARP inhibitor Response

The paper maps the genetic interaction network of PARP inhibitor response by analyzing 147,153 pairwise interactions and reveals hierarchical pathway organization and context-specific buffering interactions.

Recording morphogen signals reveals origins of gastruloid symmetry breaking

The paper shows that Wnt signaling heterogeneity predicts cell position in gastruloids, revealing symmetry breaking through signaling and cell movement.

Mapping the Genetic Landscape of DNA Double-strand Break Repair

The paper introduces Repair-seq to study DSB repair by profiling genetic perturbations and identifies dependencies and novel mechanisms in repair pathways.

Direct capture of CRISPR guides enables scalable, multiplexed, and multi-omic Perturb-seq

This paper introduces a method to directly capture CRISPR guides in single-cell RNA-sequencing libraries, enhancing Perturb-seq scalability and enabling multi-omic measurements.

Molecular recording of mammalian embryogenesis

The paper develops a molecular recorder to track lineage and define a mouse cell fate map using scRNA-seq, revealing transcriptional convergence of endodermal cells and estimating progenitor cell numbers.

Approaches to maximize sgRNA-barcode coupling in Perturb-seq screens

This paper identifies issues with barcode-sgRNA coupling in Perturb-seq and proposes solutions to improve accuracy in single-cell CRISPR screens.