Georg K. A. Hochberg

Bottom-up reconstruction of minimal pyrenoids provides insights into the evolution and mechanisms of carbon concentration by EPYC1 proteins

The paper reconstructs minimal pyrenoids using EPYC1 proteins to study their evolution and carbon concentration mechanisms.

Microcystin-Driven Control of the Carbon-Concentrating Mechanism Shapes CO2 Fixation Dynamics in Microcystis aeruginosa PCC 7806

This study shows that microcystin (MC) in Microcystis aeruginosa enhances carbon fixation by activating the carbon-concentrating mechanism (CCM) and altering photosynthetic processes under fluctuating light conditions.

Interface swapping orchestrates carbon transfer in the archaeal acetyl-CoA decarbonylase/synthase

The paper investigates the structure and dynamics of the archaeal ACDS complex, revealing a mechanism for efficient carbon transfer through transient interactions rather than stable complexes.

The anaerobic cryo-EM structure of the methanogenic Mtr complex reveals a nitrogenase-like cluster bound to its active site

The paper presents cryo-EM structures of the Mtr complex from Methanosarcina mazei, revealing a novel [Fe8S9C] cluster near the catalytic site and suggesting a link between methyl transfer and Na+ translocation.

Form IF Rubiscos include highly active, specific, and small subunit-independent enzymes.

The paper identifies Form IF Rubiscos that are highly active, specific, and function without the small subunit, challenging the necessity of subunit complexity in Rubisco evolution.

Coordination of sequential RNase activities in an ancient molecular machine

The paper studies the evolution of the RNA exosome, showing how an ancient active RNase evolved into a regulatory hub coordinating sequential RNase activities.

Drift, selection and convergence in the evolution of a nonribosomal peptide

The paper studies the evolution of nonribosomal peptides in entomopathogenic bacteria, finding evidence of both neutral drift and natural selection driving structural convergence and toxicity diversification.

Methylthio-alkane reductases use nitrogenase metalloclusters for carbon-sulfur bond cleavage

This study presents the first molecular structure of methylthio-alkane reductase, revealing metalloclusters that inform its function and evolutionary relationship to nitrogenases.

Origin of chaperone dependence and assembly complexity in Rubisco biogenesis

The paper investigates the evolutionary origin of chaperone dependence in Rubisco biogenesis and finds that this dependence evolved neutrally and can be reversed without affecting catalytic function.

Molecular model of a bacterial flagellar motor in situ reveals a "parts-list" of protein adaptations to increase torque

The paper determines the in situ structure of a high-torque bacterial flagellar motor using cryogenic electron microscopy, revealing protein adaptations and universal principles of torque generation.

A general substitution matrix for structural phylogenetics.

The paper infers a general substitution matrix for structural phylogenetics and uses it to provide evidence for a root between archaea and bacteria.

Structural insights into late-stage photosystem II assembly by Psb32

This study determines the structure of two late-stage PSII assembly intermediates, revealing how Psb32 and Psb27 regulate OEC maturation and Mn4O5Ca cluster preparation.

2-oxoglutarate triggers assembly of active dodecameric Methanosarcina mazei glutamine synthetase

This study shows that 2-oxoglutarate induces dodecamer assembly of Methanosarcina mazei glutamine synthetase, enhancing its activity through a cooperative mechanism.

Frequent transitions in self-assembly across the evolution of a central metabolic enzyme

The paper investigates the evolutionary dynamics of self-assembly in prokaryotic citrate synthases, finding frequent transitions between different assembly types without strong functional consequences.

Cardiac stress leads to regulation of Filamin C dimerisation via an ancient phosphorylation-modulated interaction with HSPB7

The paper investigates how cardiac stress regulates Filamin C dimerisation through phosphorylation-modulated interactions with HSPB7, revealing evolutionary conservation of this mechanism.

Evolution Inspired Engineering of Megasynthetases

The paper describes an evolution-inspired method to engineer NRPS by identifying a recombination breakpoint and using it to assemble NRPS fragments for producing a proteasome inhibitor.

Construction of multicellular yeast networks using the communication toolkit with variable specificity and attenuation

The paper develops a yeast communication toolkit for constructing multicellular networks with variable specificity and attenuation, demonstrating its application in logic gate-like networks and promiscuity analysis.

It takes a dimer to tango: Oligomeric small heat-shock proteins dissociate to capture substrate

The paper shows that dimeric subunits of sHsps are functional units for substrate capture, and that dimer stability affects chaperone efficiency.