Christian G. Specht

Single molecule detection of Tau seeds in extracellular vesicles from Alzheimer's disease brains

This study uses single molecule localization microscopy to detect pathological Tau proteins in extracellular vesicles from Alzheimer's disease brains.

Cryo-EM Structures of Higher Order Gephyrin OligomersReveal Principles of Inhibitory Postsynaptic Scaffold Organization

This study determines the structure of higher-order gephyrin oligomers using cryo-EM, revealing how its flexible linker regulates receptor binding and oligomerization.

Single molecule counting detects low-copy glycine receptors in hippocampal and striatal synapses

The paper uses single molecule localization microscopy to detect low-copy glycine receptors at inhibitory synapses in the hippocampus and striatum, revealing their spatial organization.

Competition between glycine and GABAA receptors for gephyrin controls their equilibrium populations at inhibitory synapses

The paper investigates competition between glycine and GABA receptors for gephyrin at inhibitory synapses using single-molecule techniques and molecular tweezers, revealing thermodynamic properties and implications for synaptic plasticity.

Identification of a stereotypic molecular arrangement of endogenous glycine receptors at spinal cord synapses

The paper quantifies the molecular arrangement of glycine receptors at spinal cord synapses, showing consistent packing densities despite morphological differences.

Differential homeostatic regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses

The paper uses super-resolution imaging to show that GlyRs and GABAARs form distinct sub-synaptic domains at mixed inhibitory synapses, with differential regulation of GABAARs under increased network activity.

Sylites: Multipurpose markers for the visualization of inhibitory synapses

The paper introduces Sylites, fluorogenic probes for visualizing inhibitory synapses with high contrast and selectivity, enabling precise nanoscopic measurements and 3D distribution analysis in brain tissue.

Reciprocal stabilisation of glycine receptors and gephyrin scaffold proteins at inhibitory synapses

This study shows GlyRs and gephyrin dynamically stabilise each other at inhibitory synapses through receptor-scaffold interactions, with GlyR crosslinking reducing gephyrin exchange but not total synaptic levels.