bioRxivNeurosciencePreprint
Sylites: Multipurpose markers for the visualization of inhibitory synapses
Vladimir KhayenkoiD, Clemens SchulteiD, Sara L. ReisiD, Orly AvrahamiD, Cataldo SchietromaiD, Rafael Worschech, Noah Frieder NordblomiD, Sonja Kachler, Carmen VillmanniD, Katrin Gertrud HeinzeiD, Andreas SchlösseriD, Ora Schueler‐FurmaniD, Philip TovoteiD, Christian G. SpechtiD, Hans Michael MariciD
Abstract
We introduce Sylites - small and versatile fluorogenic affinity probes for high-contrast visualization of inhibitory synapses. Having stoichiometric labeling and exceptional selectivity for neuronal gephyrin, a hallmark protein of the inhibitory post-synapse, Sylites enable superior synapse staining compared with antibodies. Combined with super-resolution microscopy, Sylites allow precise nanoscopic measurements of the synapse. In brain tissue, Sylites reveal the three-dimensional distribution of inhibitory synapses within just an hour.
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