Michael S. Fernandopulle

The paper introduces a high-throughput 3D culture system for imaging axonal processes in human iPSC-derived neurons, enabling efficient study of microtubule dynamics and cargo transport.

This study uses proteomic techniques to show that PGRN deficiency impairs lysosomal function and proteostasis in neurons by altering pH, enzyme levels, and protein turnover.

The paper presents a fully automated FAIMS-DIA proteomic pipeline for iPSC-derived neurons, showing high protein identification and reproducibility, validated against single-cell RNA-Seq data.

The paper develops and evaluates a proximity labeling method to study the endolysosomal proteome in human neurons, showing improved data normalization and complementary coverage compared to overexpression approaches.