Ling Hao

A Deep Quantitative Proteome Turnover Platform for Human iPSC-derived Neurons

The paper develops a platform to measure protein turnover in human iPSC-derived neurons, revealing conserved and subtype-specific proteome dynamics.

An Optimized Stem Cell Secretome Proteomics Platform: Application to Progranulin-Deficient iPSCs

This paper optimizes a secretome proteomics platform for iPSCs and applies it to study progranulin deficiency's effects on extracellular and intracellular proteomes.

Neurodegeneration risk variants promote lysosomal TMEM106B fibrilaccumulation

The paper identifies TMEM106B fibril accumulation as a shared mechanism in neurodegeneration linked to genetic variants in TMEM106B and GRN.

Interactome Analysis of the CC2D1A Scaffold Reveals Novel Neuronal Interactions and a Postsynaptic Role

This study identifies the CC2D1A interactome in human neurodevelopmental disorders, revealing synaptic roles and novel interactions in the brain.

Maintenance of neuronal TDP-43 expression requires axonal lysosome transport

The paper identifies BORC as a modifier of TDP-43 protein levels in neurons, showing lysosome transport is required for TDP-43 turnover.

CHIP protects lysosomes from CLN4 mutant-induced membrane damages

The paper identifies CHIP-mediated microautophagy as a protective mechanism against lysosomal damage caused by CLN4 mutations, showing its therapeutic potential in neurodegenerative diseases.

Multi-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient Neurons

This study uses proteomic techniques to show that PGRN deficiency impairs lysosomal function and proteostasis in neurons by altering pH, enzyme levels, and protein turnover.

Protein Contaminants Matter: Building Universal Protein Contaminant Libraries for DDA and DIA Proteomics

This study develops universal protein contaminant libraries for DDA and DIA proteomics to reduce false discoveries and improve protein identification without affecting quantification accuracy.

Integrated Proteomic and Metabolomic Analyses of the Mitochondrial Neurodegenerative Disease MELAS

This study integrates proteomic and metabolomic analyses of patient-derived fibroblasts to identify dysregulated pathways in MELAS, revealing OXPHOS defects and arginine biosynthesis downregulation.

Development and Comparative Evaluation of Endolysosomal Proximity Labeling-based Proteomic Methods in Human iPSC-derived Neurons

The paper develops and evaluates a proximity labeling method to study the endolysosomal proteome in human neurons, showing improved data normalization and complementary coverage compared to overexpression approaches.