Keean C. A. Braceros

This study shows that RSC primes quiescent chromatin for rapid transcription upon cell cycle re-entry by pre-binding target genes and facilitating Pol II loading and transcription.

The paper introduces CRISPR-Bac, a piggyBac-based system for inducible CRISPR-Cas9 genome editing in mammalian cells, enabling efficient gene knockdown, deletion, and transcriptional activation/repression.

This study shows that the SWI/SNF complex activates quiescence-specific genes by facilitating Gis1 localization and histone hypoacetylation, counteracting Rpd3-mediated repression during quiescence entry.