David M. Lee

The paper identifies how Xist Repeat A recruits SPEN via SR proteins to induce gene silencing, revealing a role for splicing factors in this process.

The paper identifies SPEN-independent transcriptional antiterminator activity in the 5' end of Xist that promotes full-length RNA production and resists silencing.

This study shows that gene conversion and NHEJ are major DNA repair mechanisms in human embryos, with gene conversion potentially useful for gene correction but also causing extensive loss of heterozygosity.

The paper introduces CRISPR-Bac, a piggyBac-based system for inducible CRISPR-Cas9 genome editing in mammalian cells, enabling efficient gene knockdown, deletion, and transcriptional activation/repression.