Megan D. Schertzer

This study uses genetics-guided targeted mass spectrometry to detect protein isoforms associated with bone mineral density, confirming their expression in clinical samples.

LRP2 is a proteogenomics pipeline that integrates long-read RNA sequencing and mass spectrometry to detect novel protein isoforms and improve their translation inference.

Isocall is a scalable method for joint transcript identification from long-read RNA-seq data, showing high precision and handling large datasets efficiently.

The paper introduces perplexity as a metric to quantify isoform diversity in the human transcriptome using long-read RNA-sequencing data, showing it provides interpretable and reproducible measurements.

The paper develops a CRISPR/Cas13d-based method to specifically knockdown RNA isoforms and provides computational tools for experimental design and analysis.

This paper introduces SPLISOSM, a framework for analyzing spatial transcriptomics data to detect isoform-level patterns, identifying spatially variable transcript diversity and regulatory relationships in mouse and human brain tissues.

The paper shows that the lncRNA Airn recruits Polycomb Repressive Complexes to silence genes during mouse embryogenesis through chromatin contacts and regulatory elements.

The paper identifies SPEN-independent transcriptional antiterminator activity in the 5' end of Xist that promotes full-length RNA production and resists silencing.

The paper introduces CRISPR-Bac, a piggyBac-based system for inducible CRISPR-Cas9 genome editing in mammalian cells, enabling efficient gene knockdown, deletion, and transcriptional activation/repression.