A chemigenetic fluorescence lifetime-based biosensor for imaging of ERK activity
Elias Dressler, Rohan Eapen, Dennis Pellanda, Yixin Zhang, Jérôme Julmi, Musadiq Ahmad Bhat, Tommaso Patriarchi, Darren T. Gilmour, Michelle S. Frei
This paper introduces a chemigenetic biosensor, WHaloEKAR, that reports ERK activity through fluorescence lifetime changes, enabling spatially resolved and multiplexed measurements in cultured cells and live animals.
bioRxiv · Oct 12, 2026 · PreprintCell Biology