Michael J. Flister

The paper introduces DICE, a CRISPR screening method that improves scalability and robustness over SLICE in THP1 cells by using dual-guide RNA infection with Cas9 electroporation.

This study screens all human paralog pairs to identify synthetic lethal interactions and finds that their strength depends on shared protein interaction networks and cellular context.

A genome-wide CRISPR screen and genetic analysis identify TNRC18 as a novel regulator of IL-1β production and a risk factor for inflammatory diseases.

This study optimizes genomewide CRISPR screens using AsCas12a and multi-guide arrays, finding that three guides per gene construct provide optimal performance.

This paper introduces padma, a pathway-centric tool that quantifies individualized multi-omic deviations in cancer, correlating them with clinical outcomes in breast and lung cancer.

The paper identifies temporally changing transcription factors and chromatin changes driving zebrafish CNS axon regeneration.

This study refines the rat genetic map to sub-centimorgan resolution using 95,769 markers and 870 meioses, revealing sex-specific recombination patterns and improving QTL localization.