Leoš Shivaya Valášek

The paper investigates the multi-layered repression system controlling C9ORF72 translation and identifies strategies to rescue loss-of-function in disease.

The paper shows that near-cognate tRNAs that promote stop codon readthrough form specific contacts with ribosomal proteins at the decoding site, with the anticodon stem length and structure influencing this interaction.

The paper shows that altering eIF3 subunit levels affects the translatome, particularly impacting MAPK signaling and ribosomal protein expression in HeLa cells.

The paper identifies a stem-loop and modified adenines in the uORF2/ATF4 overlap that regulate ATF4 translation under stress, revealing a more complex mechanism than previously thought.

The paper investigates how yeast Tma proteins affect translation reinitiation at upstream open reading frames (uORFs) and finds that their impact varies based on uORF characteristics and penultimate codons, not solely on bulk recycling dependence.

The paper investigates the readthrough-inducing potential of human tRNACys in HEK293T cells, finding that two isoacceptors can significantly elevate UGA readthrough.

The paper identifies start-stop elements in 5' UTRs that repress translation by halting ribosomes, showing their prevalence in human genes and impact on cellular processes.

The paper determines the structure of the 43S PIC from Trypanosoma cruzi and identifies structural differences in translation initiation compared to mammalian hosts.

The paper introduces Sel-TCP-seq to study initiation factor dynamics, revealing staged initiation and co-translational assembly of initiation complexes in yeast and human cells.

The paper investigates eIF3's roles in mRNA recruitment to the ribosomal preinitiation complex by analyzing functional variants in yeast, revealing distinct roles at entry and exit channels.