Sha Huang

The paper maps mesenchymal diversity in the developing human intestine and organoids using single-cell RNA-sequencing and spatial transcriptomics, identifying distinct fibroblast populations and their spatial organization.

This study develops iPSC-derived lung bud tip progenitor organoids that can be expanded and differentiated into airway or alveolar organoids, showing transcriptional similarity to in vivo progenitors.

The paper uses ChRO-seq to study chromatin dynamics during human small intestinal development, identifying enhancer activity and transcription patterns at HOX gene loci.

This study shows that non-adhesive alginate hydrogels support the growth and differentiation of human intestinal organoids, comparable to Matrigel.

This study induces and engrafts human pluripotent stem cell-derived lung bud tip progenitor-like cells in vitro and in vivo, showing their similarity to native lung tissue and multi-lineage potential.

This study uses human intestinal organoids to show microbial colonization induces complex physiological changes in immature human epithelium, including antimicrobial peptide production and mucus maturation.