Sebastiaan van Heesch

The paper shows EWSR1::FLI1 fusion drives the production of cancer-specific neoproteins in Ewing sarcoma, revealing a reversible dark proteome with therapeutic potential.

The paper identifies 25% of 7,264 non-canonical open reading frames (ncORFs) as producing translated gene products, supported by over 3,000 peptides from 3.8 billion mass spectra.

The paper expands and refines a reference catalog of human non-canonical open reading frames (ncORFs) by incorporating more datasets and introducing a translation signature score framework to prioritize high-quality ncORFs.

This study identifies LINC01503-MP, a mitochondrial microprotein derived from an lncRNA, which promotes cell proliferation and oxidative metabolism in colorectal cancer cells.

The paper assesses MS-based identifications of unannotated human microproteins, finding large variation in reported proteins and PSM quality across studies.

This paper discusses the current state of research on non-canonical open reading frames (ORFs) and proposes standards for their investigation and reporting using Ribo-seq and proteomics.

This study investigates the role of RPL3L in ribosome heterogeneity and mitochondrial activity in cardiomyocytes, finding that its depletion increases ribosome-mitochondria interactions and ATP levels.

This paper outlines a community-driven initiative to create a consolidated catalog of human Ribo-seq ORFs, addressing the lack of standardized annotation for these features.

The paper identifies 27 RBPs that regulate both mRNA abundance and translational efficiency in human hearts, with 21 affecting distinct sets of target genes through differential affinity for target length.

The paper uses CAGE sequencing to identify alternative promoter usage in a rat model of hypertension, linking promoter shifts to insulin resistance and blood pressure.

The paper identifies a trans locus in rats that causes a protein length-dependent shift in translational efficiency, affecting cardiac hypertrophy and sarcomere protein stoichiometry.

The paper identifies a microprotein-independent role for LINC00261 in endocrine cell differentiation during human pancreas development through genetic ablation of lncRNAs.

The paper identifies translational regulators of fibrogenic genes in human cardiac fibroblasts and shows their role in cardiac fibrosis through ribosome profiling and RNA-binding protein analysis.

The paper identifies Tug1 as essential for male fertility, showing it has three distinct regulatory activities: a cis DNA repressor, a trans-acting lncRNA, and a conserved peptide affecting mitochondrial function.