Shintaro Yamada

BRCA2 loss triggers a downward spiral of genomic instability via ROS-dependent metabolic collapse

This paper shows BRCA2 loss causes genomic instability through ROS-dependent metabolic collapse, highlighting the role of glutathione metabolism and PARP inhibitors in cell death.

Reconstitution of SPO11-dependent double-strand break formation

This study reconstitutes SPO11-dependent DNA double-strand break formation in vitro, revealing dimerization-dependent cleavage and topoisomerase activity.

The MRE11-RAD50-NBS1 complex both starts and extends DNA end resection in mouse meiosis

This study shows the MRN complex is essential for both initiating and extending DNA end resection in mouse meiosis, with defects leading to spermatogenic failure.

Optimized methods for mapping DNA double-strand-break ends and resection tracts and application to meiotic recombination in mouse spermatocytes

The paper optimizes S1-seq for mapping meiotic DSBs and resection tracts in mouse spermatocytes and evaluates quantitative features of resection.

TDP 2 modulates the expression of estrogen-responsive oncogenes

This study shows that TDP2 regulates estrogen-induced oncogene expression by resolving TOP2-induced DNA breaks, affecting MYC and CCND1 in ER-positive cells and mice.

Triple-helix potential of the mouse genome

This study uses S1-seq to evaluate H-DNA triplex-forming potential in the mouse genome, identifying motifs and structural features associated with DNA secondary structure.

ATM and PRDM9 Regulate SPO11-bound Recombination Intermediates During Meiosis

This study directly detects meiotic DSBs and resection in vertebrates using END-seq on mouse spermatocytes, identifying a novel recombination intermediate regulated by ATM and PRDM9.

Molecular structures and mechanisms of DNA break processing in mouse meiosis

The paper defines resected DSB structures in mouse spermatocytes, identifies ATM as a key regulator of resection, and shows PRDM9-dependent chromatin remodeling at recombination sites.

Genomic and chromatin features shaping meiotic double-strand break formation and repair in mice

This study investigates the genomic and chromatin features influencing meiotic DSB formation and repair in mice, identifying roles for PRDM9 and ATM in hotspot activity and recombination outcomes.

A global view of meiotic double-strand break end resection

The paper maps meiotic resection endpoints genome-wide in yeast, revealing heterogeneity and the role of Exo1 and Tel1 in resection initiation and processivity.