Truc T. Losier

This paper introduces a refined CRISPR screening protocol to compare cellular responses across multiple stressors, reducing false positives by 40% through fluorescence-based reporters and fixation steps.

The paper identifies stress-specific autophagy regulators using CRISPR screens, finding unique kinases for ER-phagy and pexophagy.

This study shows ULK1 phosphorylates ATG16L1, enhancing wildtype function but destabilizing the Crohn’s disease-associated mutant, thereby affecting xenophagy.