Pierre Affaticati

The paper develops a multiplex CRISPR-Cas9 gRNA toolkit to generate fully unpigmented zebrafish larvae with minimal mortality, enabling improved imaging without chemical inhibitors.

The paper identifies SIRanc, a human homolog of bacterial antiphage SIR2 proteins, and shows it mediates innate immunity via the TLR pathway by degrading NAD+.

The paper shows that modulating Embryonic Neuronal Excitability (ENE) in zebrafish embryos affects dopaminergic neuron numbers and larval locomotion, with implications for neurodevelopmental disorders.

ZeBraInspector enables high-content 3D imaging and volumetric analysis of zebrafish brain white matter to characterize phenotypes and treatment effects.

The paper investigates the developmental origin of a visual relay nucleus in zebrafish, finding it arises from the midbrain rather than the forebrain, suggesting non-thalamic evolution of visual pathways.