The paper develops a multiplex CRISPR-Cas9 gRNA toolkit to generate fully unpigmented zebrafish larvae with minimal mortality, enabling improved imaging without chemical inhibitors.

bioRxiv · Oct 28, 2026 · PreprintMolecular Biology

Pdgfrβ signaling orchestrates meningeal repair via the mobilization of arachnoid cells.

Payel Banerjee, Michaël Demarque, Axel Benchetrit, Dorian Champelovier, Cynthia Froc, Manon Paul, Hannah Wiggett, Arnim Jenett, Jean‐Pierre Levraud

This study identifies PDGFRβ signaling as critical for meningeal cell recruitment and tissue remodeling during zebrafish CNS injury repair.

bioRxiv · May 15, 2026 · PreprintCell Biology

The two groups of zebrafish type I interferons target different tissues, paralleling the mammalian type I: type III IFN functional division

Hannah Wiggett, Dean Porter, Valérie Briolat, Teodosia Balaboi, Maroun Abi Younes, Ingrid Colin, Georges Lutfalla, Jean‐Pierre Levraud

This study shows zebrafish type I interferons target different tissues, mirroring the mammalian division between type I and type III IFNs.

bioRxiv · Nov 3, 2025 · PreprintImmunology

A human homolog of SIR2 antiphage proteins mediates immunity via the TLR pathway

Delphine Bonhomme, Hugo Vaysset, Eirene Marie Q. Ednacot, Vasco Rodrigues, Yazan Salloum, Jean Cury, Anny Wang, Axel Benchetrit, Pierre Affaticati, Veronica Hernandez Trejo, Paul Vittot, Charlie Bories, Alexis Cornec, Jean‐Pierre Levraud, Pedro P. Hernández, , Enzo Z. Poirier

The paper identifies SIRanc, a human homolog of bacterial antiphage SIR2 proteins, and shows it mediates innate immunity via the TLR pathway by degrading NAD+.

Science · Jul 24, 2025Peer-reviewedImmunology

Interleukin-22 in enteroendocrine cells controls early-life gut motility through interactions with the microbiota

Soraya Rabahi, Emiliano Marachlian, Fabian Guendel, Aya Mikdache, Keinis Quintero-Castillo, Lucie Maurin, Vincenzo Di Donato, Akshai Janardhana Kurup, Yazan Salloum, Gwendoline Gros, Patricia Diabangouaya, Camila Garcia-Baudino, Ignacio Medina-Yáñez, Jean‐Pierre Levraud, Georges Lutfalla, , Pedro P. Hernández

This study shows that IL-22 from enteroendocrine cells in zebrafish larvae controls gut microbiota and motility, with similar effects observed in mice.

bioRxiv · Apr 25, 2025 · PreprintImmunology

Mapping the journey of a neuro-invasive virus in zebrafish larvae: contrasted viral spread and type I interferon responses in periphery and central nervous system

Valerio Laghi, Laurent Boucontet, Hannah Wiggett, Payel Banerjee, Matthieu Simion, Ludovico Maggi, Sorana Ciura, Jérémie Guedj, Emma Colucci‐Guyon, Jean‐Pierre Levraud

This study tracks SINV spread in zebrafish larvae, showing CNS entry via neurons, persistent CNS infection, and differential IFN responses, with a mathematical model revealing IFN's dual role in infection control.

bioRxiv · May 19, 2024 · PreprintMicrobiology

Plasticity of the dopaminergic phenotype and of locomotion in larval zebrafish induced by changes in brain excitability during the embryonic period

Sandrine Bataille, Hadrien Jalaber, Ingrid Colin, Damien Remy, Pierre Affaticati, Cynthia Froc, Jean‐Pierre Levraud, Philippe Vernier, Michaël Demarque

The paper shows that modulating Embryonic Neuronal Excitability (ENE) in zebrafish embryos affects dopaminergic neuron numbers and larval locomotion, with implications for neurodevelopmental disorders.

eNeuro · Jun 1, 2023Peer-reviewedNeuroscience