Author

Daniela Nicastro

The University of Texas Southwestern Medical CenterORCID 0000-0002-0122-7173Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

Click to filter author papers

Cell Biology· 2 Biophysics· 1 Molecular Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:cell type annotation· 3 Approach:experimental assay· 3 Assay:cryo-EM· 2 Approach:deep learning· 2 Organism:mouse· 2 Assay:fluorescence / live-cell microscopy· 1 Research Focus:protein folding & dynamics· 1 Research Focus:protein structure prediction· 1

Frequent Co-Authors

Daniel StoddardiD· 2 shared Kangkang SongiD· 2 shared Amirrasoul TavakoliiD· 1 shared Amy S. FabritiusiD· 1 shared Angeles Fernandez-Gonzalez· 1 shared Bingying CheniD· 1 shared Bo-Jui ChangiD· 1 shared Brian A. BaylessiD· 1 shared

Activity by Year

2018-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 16 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

2 shared papersCell Biology

Daniel StoddardORCID

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy

Seweryn Gałecki, Bo-Jui Chang, Felix Zhou, Qionghua Shen, Daniel Stoddard, Bingying Chen, Daniela Nicastro, Reto Paul Fiolka, Kevin M. Dean

This paper introduces Cy-ExM, a high-plex imaging strategy that enables 3D super-resolution mapping of cellular ultrastructure in mammalian cells.

bioRxiv · Jan 2, 2026 · PreprintCell Biology

The cryo-EM structure of mouse radial spoke 3 reveals a unique metabolic and regulatory hub in cilia

Yanhe Zhao, Kangkang Song, Amirrasoul Tavakoli, Long Gui, Angeles Fernandez-Gonzalez, Song Zhang, Petras P. Dzeja, S. Alex Mitsialis, Xuewu Zhang, Daniela Nicastro

The paper determines the 3D structure of mouse radial spoke 3 (RS3) using cryo-EM and identifies its role as a metabolic and regulatory hub in ciliary motility.

Nature Structural & Molecular Biology · Jun 27, 2025Peer-reviewedBiophysics

Proteomic analysis of microtubule inner proteins (MIPs) in Rib72 null Tetrahymena cells reveals functional MIPs

Amy S. Fabritius, Brian A. Bayless, Sam Li, Daniel Stoddard, Westley Heydeck, Christopher C. Ebmeier, Lauren Anderson, Tess Gunnels, Chidambaram Nachiappan, Justen B. Whittall, William M. Old, David A. Agard, Daniela Nicastro, Mark Winey

The paper identifies and functionally characterizes MIPs lost in RIB72A/B knockout Tetrahymena cells using proteomics and cryo-electron tomography.

Molecular Biology of the Cell · Aug 18, 2021Peer-reviewedMolecular Biology

Structure of the ciliary axoneme at nanometer resolution reconstructed by TYGRESS

Kangkang Song, Zhiguo Shang, Xiaofeng Fu, Xiaochu Lou, Nikolaus Grigorieff, Daniela Nicastro

A hybrid cryo-electron microscopy method reveals subcellular structures at unprecedented resolution.

bioRxiv · Jul 6, 2018 · PreprintCell Biology