Reto Paul Fiolka

Deciphering the Multiscale Morphology of Somatic Oncogenic Alterations in Hepatocellular Carcinoma

This study maps how distinct oncogenic drivers induce unique multiscale architectural changes in liver tissue, revealing mutation-specific remodeling across cellular and mitochondrial structures.

Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging

Altair-dvOPM is an open-access microscope for large-field 3D tissue imaging, achieving micrometer resolution without custom objectives or specialized alignment.

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions

This study shows that branched actin-driven membrane protrusions (BAMPs) enhance oncogenic KRAS signaling by amplifying upstream regulators and promoting cell proliferation through merlin inactivation.

Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy

This paper introduces Cy-ExM, a high-plex imaging strategy that enables 3D super-resolution mapping of cellular ultrastructure in mammalian cells.

Universal consensus 3D segmentation of cells from 2D segmented stacks

The paper introduces u-Segment3D, a tool that converts 2D cell segmentations into 3D consensus segmentations without training data, demonstrating effectiveness across diverse datasets.

Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet Fluorescence Microscopy.

The paper introduces miOPM, a reconfigurable LSFM platform that enables high-resolution imaging across multiple spatial scales and refractive indices.

A deconvolution solution to aliasing confusion in apparently undersampled oblique plane microscopy

The paper introduces a deconvolution method to reconstruct properly sampled volumes from apparently undersampled oblique plane microscopy data, improving upon a previous Fourier-stitching approach.

Increasing the acquisition speed in oblique plane microscopy via Aliasing

This paper demonstrates that controlled aliasing in oblique plane microscopy can increase acquisition speed without loss of resolution or artifacts, enabling faster subcellular 3D imaging of mitochondrial dynamics.

Active Remote Focus Stabilization in Oblique Plane Microscopy

This paper introduces a method for stabilizing the light-sheet in oblique plane microscopy without using sample fluorescence, achieving high axial precision for long-term imaging.

Caveolin-1 regulates context-dependent signaling and survival in Ewing Sarcoma.

This study identifies Caveolin-1 as a context-dependent regulator of survival signaling in Ewing Sarcoma, revealing a reversible CD99High state with enhanced survival.

High-Resolution, Large Imaging Volume, and Multi-View Single Objective Light-Sheet Microscopy

This paper introduces a novel single objective light-sheet microscope that achieves high resolution, large field of view, and multi-view imaging for various biological systems.