Nikolaus Grigorieff

This paper improves cryo-EM reconstruction of small complexes using 2D template matching and high-resolution structures as priors, enhancing density of ligand-binding sites in a 43 kDa protein kinase.

This study reveals how eIF5-mimic proteins regulate start codon selection through structural insights from cryo-EM and biochemical assays.

Unbend improves cryo-EM image correction by addressing localized sample motion with a 3D spline model, enhancing signal-to-noise ratio and particle detection.

The paper observes decreased molecular crowding during yeast to filamentous growth transition in C. albicans, linked to reduced ribosome concentration and increased cytoplasmic volume.

This study shows that fluconazole tolerance in fungi is linked to decreased cytoplasmic fluidity and increased heterogeneity, mediated by changes in ribosome dynamics and P-body formation.

The paper uses in extracto cryo-EM to study ribosome hibernation factors, finding eEF2 as the most abundant hibernation factor bound to 60S and 80S ribosomes.

The paper presents a cryo-EM conformational heterogeneity challenge to evaluate and improve methods for analyzing molecular motions in heterogeneous samples.

The paper compares CEMOVIS and FIB-milling for cryo-EM sample preparation, showing CEMOVIS provides more imageable area with comparable structural preservation.

This paper introduces cryoSBI, a method using simulation-based inference to infer biomolecular conformations from cryo-EM images, achieving fast and accurate results.

This study uses cryo-electron tomography to show that sialic acid binding causes influenza HA trimers to cluster and form triplets on virions.

This paper introduces a new statistical metric, 2DTM p-value, to improve target detection in cryo-EM by combining SNR and z-score, showing better performance for complex targets.

CTFFIND5 improves CTF estimation by incorporating sample thickness and tilt, enhancing cryoEM image quality assessment.

The paper reports the structure of vesicular stomatitis virus L protein in complex with its phosphoprotein cofactor, revealing how P induces a closed conformation for RNA synthesis.

The paper determines the in situ structure of rotavirus VP1 RNA-dependent RNA polymerase using local reconstruction, showing conformational changes during transcription and stochastic distribution of VP1 molecules.

A hybrid cryo-electron microscopy method reveals subcellular structures at unprecedented resolution.