Fabio Giuseppe Liberante

Generating long deletions across the genome with pooled paired prime editing screens

This paper develops a pooled paired prime editing screen to generate large-scale deletions across the genome, identifying non-coding regions with fitness effects and demonstrating the feasibility of genome-wide deletion perturbation.

Randomizing the human genome by engineering recombination between repeat elements

This paper develops a CRISPR-based method to generate large-scale structural genomic rearrangements in human cells, revealing selection pressures and gene expression impacts of these changes.

Resolution of a human super-enhancer by targeted genome randomisation

The paper uses enhancer scrambling to study a super-enhancer of the OTX2 gene, finding that a single DNase I hypersensitive site predominantly controls its expression.

Predicting efficiency of writing short sequences into the genome using prime editing

The paper predicts insertion efficiency of short sequences into the genome using prime editing by analyzing sequence features and developing a machine learning model.