Marcin Warmiński

RNA demethylase FTO uses conserved aromatic residues to recognize the mRNA 5' cap and promote efficient m6Am demethylation

This study identifies conserved aromatic residues in FTO that recognize the mRNA 5' cap and enhance m6Am demethylation, providing structural insights into FTO's selectivity for m6Am over m6A.

FTO separation-of-function mutations alter m6A versus m6Am demethylation selectivity on RNA

This paper reports engineered FTO mutants that selectively demethylate m6A or m6Am RNA modifications, enabling study of their distinct biological roles.

The trypanosome mRNA decapping enzyme ALPH1 prefers caps without m7G methylation and produces diphosphate-RNA

This study characterizes the trypanosome mRNA decapping enzyme ALPH1, showing it preferentially cleaves non-m7G-capped RNAs and produces diphosphate-RNA.

Chemical circularization of in vitro transcribed RNA opens new avenues for circular mRNA design

This paper introduces a chemical method to circularize in vitro transcribed RNA, producing chemically circularized RNAs (chem-circRNAs) with high efficiency and compatibility with modifications.

How ascorbate tunes the activity and substrate selectivity of Fe(II)-dependent dioxygenase superfamily enzymes

This study reveals how ascorbate regulates FODD enzyme activity and substrate selectivity, providing structural insights into its role in RNA demethylation and broader biological oxidation reactions.

An MST-based assay reveals new binding preferences of IFIT1 for canonically and non-canonically capped RNAs

This study develops an MST-based assay to assess IFIT1 binding to various capped RNAs, revealing new preferences for canonical and non-canonical RNA caps.

Structural basis of Spliced Leader RNA recognition by the Trypanosoma brucei cap-binding complex

This paper reports cryo-EM structures of the Trypanosoma brucei cap-binding complex (TbCBC) and identifies its interactions with the spliced leader RNA.