This study shows that external and internal gills in amphibians both originate from a continuous pharyngeal endoderm epithelium, resolving a long-standing developmental question.

bioRxiv · Aug 12, 2026 · PreprintDevelopmental Biology

NOTCH1-specific phosphorylation of S1970 by Casein Kinase 1 is required for NOTCH1 transcriptional competence and signaling activity in vivo

Fabio Turetti, Lorena Agostini Maia, Hana Hajsmanova, Jakub Sławski, Wim Dehaen, Marek Dokoupil, Giovanna M. Collu, Tomáš Gybeľ, Kamil Paruch, Emma Rachel Andersson, Vı́tězslav Bryja, Pavla Perlíková, Konstantinos Tripsianes, Jakub Harnoš, Jan Mašek

This study identifies CK1-mediated phosphorylation of NOTCH1 at S1970 as essential for its transcriptional activity and signaling in vivo.

bioRxiv · May 7, 2026 · PreprintCell Biology

Armed with PRICKLE(3)s: Stabilizing WNT/PCP complexes against RNF43-mediated ubiquitination

Katarzyna Anna Radaszkiewicz, Tomasz Witold Radaszkiewicz, Pavla Kolarova, Petra Paclíková, Kristína Gömöryová, Tomáš Bárta, Kateřina Hanáková, Zbyněk Zdráhal, Jakub Harnoš

This study identifies PRICKLE3's role in stabilizing WNT/PCP complexes by inhibiting RNF43-mediated ubiquitination of VANGL1/2.

bioRxiv · Mar 24, 2025 · PreprintCell Biology

Unveiling Vertebrate Development Dynamics in Frog Xenopus laevis using Micro-CT Imaging

Jakub Laznovsky, Michaela Kavková, Alice H. Reis, Pavla Robovská-Havelková, Jan Křivánek, Tomáš Zikmund, Jozef Kaiser, Marcela Buchtová, Jakub Harnoš

This paper uses micro-CT imaging to generate a comprehensive dataset of Xenopus laevis development, capturing morphological changes from tadpoles to adults for further analysis.

bioRxiv · Jun 11, 2024 · PreprintDevelopmental Biology