Stefan H. Mueller

Fluorescence cross-correlation spectroscopy quantifies affinity, cooperativity, and kinetic stability in ternary protein complexes

This paper uses FCCS to quantify affinity, cooperativity, and kinetic stability in ternary protein complexes, showing cooperative stabilization of the HER2-trastuzumab-pertuzumab complex.

The interplay between supercoiling and DNA modifying enzymes at the single-molecule level

This study investigates how DNA supercoiling affects the activity of the restriction enzyme EcoRV at the single-molecule level using TIRF microscopy.

DNA barcoding for parallelised single-molecule characterisation of kinetic phenotypes

This paper introduces a DNA barcoding method to simultaneously profile kinetic properties of protein variants at the single-molecule level, revealing kinetic diversity and structural subpopulations.