Andrew D. Griffiths

DNA barcoding for parallelised single-molecule characterisation of kinetic phenotypes

This paper introduces a DNA barcoding method to simultaneously profile kinetic properties of protein variants at the single-molecule level, revealing kinetic diversity and structural subpopulations.

Optimised Nanobody-based Quenchbodies for Enhanced Protein Detection

This study optimizes nanobody-based quenchbodies for enhanced protein detection, achieving fluorescence increases of 1.5-2.4-fold and detecting IL-6 down to 2 nM.

Parallelization of single-molecule binding kinetic measurements via protein barcode sequencing

This paper introduces a method to parallelize single-molecule binding kinetic measurements using protein barcode sequencing, enabling rapid and multiplexed screening of protein libraries.

Global regulators facilitate adaptation to a phenotypic trade-off

The paper shows global regulators enable adaptation to a growth-motility trade-off in E. coli by modulating both phenotypes, while local regulators mainly affect motility.

The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands.

This study uses scRNA-seq to analyze Trypanosoma brucei development in tsetse fly salivary glands, revealing dynamic VSG gene activation and monoallelic expression establishment.

Metabolic Cost of Rapid Adaptation of Single Yeast Cells

The paper demonstrates metabolic coupling during rapid adaptation of single yeast cells by measuring metabolism and division in thousands of individual cells.