Timothy D. Craggs

DNA damage induces long range changes to duplex structure - a non-protein start to damage detection?

This study shows DNA damage alters duplex structure and dynamics, potentially signaling repair protein recruitment through changes in DNA flexibility.

A protein-dependent riboswitch activates ribosomal frameshifting in cardioviruses

The paper investigates how the 2A protein interacts with the RNA genome of cardioviruses to activate ribosomal frameshifting through structural changes in the RNA.

Fluorescence cross-correlation spectroscopy quantifies affinity, cooperativity, and kinetic stability in ternary protein complexes

This paper uses FCCS to quantify affinity, cooperativity, and kinetic stability in ternary protein complexes, showing cooperative stabilization of the HER2-trastuzumab-pertuzumab complex.

The interplay between supercoiling and DNA modifying enzymes at the single-molecule level

This study investigates how DNA supercoiling affects the activity of the restriction enzyme EcoRV at the single-molecule level using TIRF microscopy.

A universal protein ladder for standardisation of diverse FRET assays

The paper introduces a universal protein ladder to standardize FRET assays across different platforms and experimental conditions.

RNA i-Motif Formation at Neutral pH

This study investigates RNA i-motif stability at neutral pH, finding that while ensemble experiments show global unfolding, single-molecule experiments reveal a small fraction remains folded.

CBP-IDRs regulate acetylation and gene expression.

This study shows how different IDRs in CBP regulate its condensate properties, affecting gene expression and histone acetylation.

Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo

This study investigates the conformational flexibility of kinesin-1 using biophysical methods, revealing its open structure without cargo and insights into autoinhibition.

The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states

This study investigates the conformational transitions of Rep helicase using single-molecule techniques, revealing two intermediate states influenced by salt concentration.

Structural basis of multitasking by the apicoplast DNA polymerase from Plasmodium falciparum

This study determines the structural basis of multitasking by the apicoplast DNA polymerase from Plasmodium falciparum using cryoEM, revealing how it balances replicative and lesion bypass synthesis.

A DNA scaffold approach facilitates 5' labelling of the SARS-CoV-2 RNA pseudoknot for smFRET investigation

This paper develops a DNA scaffold method for 5' labeling of SARS-CoV-2 RNA pseudoknots to study their conformational dynamics using smFRET.

G-Quadruplexes Act as an On/Off Switch While i-Motifs Regulate Insulin Expression in Reporter Gene Assays

This study shows that both G-quadruplexes and i-motifs are required for glucose-induced transcriptional activation of the insulin gene, with i-motif stability positively correlating with promoter activity.