Jonnathan Singh Alvarado

Matching cell masks between two-photon calcium images and thick, expanded EASI-FISH volumes recovers molecular identity for 72% (slice) to 83% (in vivo) of imaged neurons on conventional microscopes.

This paper develops a red fluorescent calcium indicator, SomaFRCaMPi, which improves neuronal activity imaging accuracy and scale in vivo by reducing neuropil contamination and enhancing signal-to-noise ratio.

The paper shows that transient cAMP production in brainstem parabrachial neurons suppresses feeding for minutes, matching the duration of cAMP and calcium activity elevations.

The paper investigates noradrenergic projections from the locus coeruleus to the cortico-basal ganglia system in zebra finches, finding regionally specialized receptor expression and divergent connectivity.

MIN1PIPE is a pipeline for extracting neuronal signals from 1-photon miniscope calcium imaging data, showing superior efficiency and precision in noisy conditions.