Crystian I Massengill

EASI-PASS: An accessible pipeline for linking functional imaging and mRNA profiling

Matching cell masks between two-photon calcium images and thick, expanded EASI-FISH volumes recovers molecular identity for 72% (slice) to 83% (in vivo) of imaged neurons on conventional microscopes.

Simultaneous, real-time tracking of many neuromodulatory signals with Multiplexed Optical Recording of Sensors on a micro-Endoscope

This paper develops a micro-endoscope probe to simultaneously track multiple neuromodulators in real time using fluorescent sensors, demonstrating its use in brain slices and awake mice.

Transient cAMP production drives rapid and sustained spiking in brainstem parabrachial neurons tosuppress feeding

The paper shows that transient cAMP production in brainstem parabrachial neurons suppresses feeding for minutes, matching the duration of cAMP and calcium activity elevations.

Highly sensitive genetically-encoded sensors for population and subcellular imaging of cAMP in vivo

The paper develops improved genetically-encoded cAMP sensors, cAMPFIREs, for high-sensitivity in vivo imaging of cAMP dynamics in neurons and Drosophila.