The paper identifies trans- and cis-COM domains in NRPS systems that mediate enzyme communication, revealing a unified model for maintaining assembly line fidelity in glycopeptide antibiotic biosynthesis.

bioRxiv · Sep 11, 2026 · PreprintMicrobiology

A searchable metadata network graph for microbiome metabolomics

Vincent Charron‐Lamoureux, Shipei Xing, Abubaker Patan, Corinn Walker, Ricardo Almada‐Monter, Yasin El Abiead, Haoqi Nina Zhao, Lucas Patel, Yuhan Weng, Antonio Gonzalez, Gail L. Ackermann, Victoria Deleray, V. Kalai Gandhi, Ipsita Mohanty, Andrés Mauricio Caraballo‐Rodríguez, , Pieter C. Dorrestein

The paper introduces microbiomeMASST, a metadata network graph linking metabolites across datasets, enabling cross-study analysis of microbial metabolites and their biological context.

bioRxiv · Feb 5, 2026 · PreprintBiochemistry

Identification of the negamycin split biosynthetic gene cluster in Kitasatospora purpeofusca ATCC21470

Alena Strüder, Saad Touzi, Mayarasmi Long, Erik Mingyar, Carolin Schwitalla, Anne Berscheid, Sophie Bourcier, Heike Brötz‐Oesterhelt, Emmanuelle Darbon, Paul Frankenreiter, Mirita Franz‐Wachtel, Christian Geibel, Theresa Anisja Harbig, Chambers C. Hughes, Boris Maček, , Evi Stegmann

This study identifies and characterizes the negamycin biosynthetic gene cluster in Kitasatospora purpeofusca, enabling scalable production through heterologous expression.

bioRxiv · Jul 31, 2025 · PreprintMicrobiology

Structural and evolutionary analyses support reclassification of glycopeptide antibiotics as xyclopeptides

Athina Gavriilidou, Noel Kubach, Martina Adamek, Jens-Peter Rodler, Susanna Kramer, Daniel H. Huson, Rosa Alduina, Gerard D. Wright, Mohammad Seyemsayadost, Wolfgang Wohlleben, Stefano Donadio, Margherita Sosio, Min Xu, Max J. Cryle, Evi Stegmann, Nadine Ziemert

The paper reclassifies glycopeptide antibiotics as xyclopeptides based on structural and evolutionary analyses of biosynthetic gene clusters.

bioRxiv · Feb 10, 2023 · PreprintGenetics