Stefano Donadio

S9 Protease WprP Catalyzes Uniform and Sequential Cleavage on the Precursor Peptide in RiPP Biosynthesis

The paper identifies WprP2 as a serine protease that uniformly and sequentially cleaves the precursor peptide WprA2 in RiPP biosynthesis, expanding the known functions of S9 proteases.

The Molecules Gateway: a homogeneous, searchable database of 150k annotated molecules from Actinomycetes

The paper introduces the Molecules Gateway, a database of 150,000 annotated molecules from Actinomycetes, enabling rapid identification of microbial products and analysis of chemical diversity.

Structural and evolutionary analyses support reclassification of glycopeptide antibiotics as xyclopeptides

The paper reclassifies glycopeptide antibiotics as xyclopeptides based on structural and evolutionary analyses of biosynthetic gene clusters.

Structural basis of transcription inhibition by fidaxomicin (lipiarmycin A3)

This paper reports the cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme bound to fidaxomicin, revealing its unique binding site and mechanism of transcription inhibition.

Antibacterial nucleoside-analog inhibitor of bacterial RNA polymerase: pseudouridimycin

This paper discovers pseudouridimycin, a novel antibacterial compound that inhibits bacterial RNA polymerase and shows potent activity against drug-resistant pathogens.